To follow NS3-catalysed unwinding, we flow NS3 (1–90 nM) and ATP (0.05–1 mM) together in buffer U (see Methods). We then hold the RNA substrate at a constant force of between 5 and 17 pN. NS3 loads on ...
The recent outbreak of Zika virus (ZIKV) infections in the Americas represents a serious threat to the global public health. The viral protease that processes viral polyproteins during infection ...
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